3D Bioactive Alkyl Peptides Scaffold to Conduct Neural Differentiation

Background and Aim: Biocompatible and non-immunogenic materialslike peptides are a great candidate for 3D scaffolds construction intissue engineering. Self-assembling alkyl peptides which can constructnanofibers in at this category of materials. There is the possibility offunctionalizing of the scaffolds by bioactive peptides for various propertieslike differentiation. Here, a functionalized alkyl peptide with neuraldifferentiating properties has been taken to investigate its fiber formationand differentiation activity. The FAQRVPPEEEGGGAAAAK(C16) is thealkyl peptide sequence which FAQRVPP plays neural differentiation roleand the rest of the sequence applied for different properties.Methods: we considered two different systems composing of just allfunctionalized alkyl peptides and a combination of functionalized andnon-functionalized (EEEGGGAAAAK(C16)) system with 1:2 ratios. Thelater one could help to have a less condensation of epitopes of the fibersurface and more stability of fiber and accessibility of epitopes. So byconsideration of previously computational study of fiber formationfrom alkyl peptides they were synthesized and analyzed by CD, FTIRspectroscopy, and TEM imaging.Results: The CD and FTIR results showed that in the combined systemmore beta structure has formatted which can verify more prepotencyof fiber construction of this system than the all functionalized system.Beside TEM image visualized the nanofibers with 8 and 10 nm indiameters for all functionalize and combined system respectively. Thisfibrous structure also formed a 3D scaffold in hydrogel networks to beused for cell culture.Conclusion: To summarize two different types of nanofiber have beenformed. The combined system showed a more cylindrical fiber typewith more width and less length and the all-functionalized system havea lesser width and higher length. these two different types of fiber canaffect the differentiation efficacy and mechanical interaction of cellsthose surfaces.

کلیدواژه ها:

Bioactive scaffolds ، Alkyl peptides ، Self-assembling nanofiber ، Amphiphilic peptides