The Panel of Circular RNAs and Target Gene Expression as a Biomarker to Early Detection of Gastric Cancer Based on Bioinformatics Methods

Background and Aim: Gastric cancer (GC) is one of the most commoncancers worldwide Gastric cancer is a multi-factorial and multi-stepprocess that involves significant molecular participation and complexnetwork regulations. Advancements in molecular medicine helppredict the possibility of the recurrence of late-stage gastric cancer withmolecular markers effectively. In a series of molecular markers, circularRNAs (circRNA) provides several advantages. Tumor-suppressing andoncogenic functions of circRNA plays a strong regulatory function incarcinoma. In this study, we aimed to identify target genes and specificbiomarkers for the identification and treatment of GC.Methods: In this bioinformatic study, all of the circRNAs were collectedfrom the literature studies that they were screened based on highthroughput technics like RNAseq and microarray screening of normaland cancer sample of patients with GC. The gene expression profilesof GC were obtained from Gene Expression Omnibus (GEO) (availableat http://www.ncbi.nlm.nih.gov/geo/) database, which included 20gastric cancer tissue samples from previously untreated patients and20 normal gastric tissue samples. The following keywords and theircombinations were used: homo sapiens and Gastric cancer. The originalstudies that compared gene expression profiling between GC and normal control (NC) biopsy tissues or cultured cells were included inthis study. Nonhuman studies and reviews were deleted. Gastric cancergene expression profiles of the GSE27342 and GSE22804 datasets weredownloaded from the GEO.Results: Analysis of circRNA database: After completing the finalanalysis, we collected 30 valuable circRNA includes the 15 upregulatedand 15 downregulated such as Has-circ-0080517, Has-circ-0000278,Has-circ-0026674 and Has-circ-0054220 as a novel panel of biomarkersto detect GC at the early stage. All these advice panels of biomarkerswere evaluated in the Cancer Specific CircRNA Databases . Accordingto the limited number of studies regarding the role of CircRNA in GCmore studies will be needed in the future to demonstrate the importanceof CircRNA in tumor initiation, invasion, and metastasis, as well astheir utility as therapeutic targets. Differentially expressed genes (DEGs)and circRNAs (DECs) in the integrated analysis of microarray datasets:After the electronic search, there were 42 microarray studies obtainedaccording to the inclusion criteria. A total of 3327 DEGs; 1330 of whichwere downregulated and 1997 of which were upregulated, and 50 DECs,including 5 down and 45 upregulated, were identified in GC samplescompared with healthy gastric tissues.Conclusion: Gastric cancer was the leading cause of cancer-relatedmortality worldwide. In recent years, many studies have discovered thelink between biomarkers and gastric cancer in the process of tumorigenicand tumor progression, some of which suggest that circRNA may serveas new biomarkers for GC patients. The most consistently overexpressedgenes of CXXC5, NOTCH1, MALAT1, MYH9, and other genes mightparticipate in the pathology of GC, and they will be the importantbiomarkers for the diagnosis of GC and in future studies, this panel willbe generalizable.

کلیدواژه ها:

Gastric cancer ، Homo sapiens ، Bioinformatic and CircRNAs