Mesenchymal Stem Cell and Electromagnetic Field

Background and Aim: Wharton’s jelly-derived mesenchymal stem cells(WJ-MSCs) are a population of stem cells in umbilical cords Wharton’sjelly that can be a notable therapy option for tissue engineering andregenerative medicine due to lack of tumorigenicity and much moreimmunosuppressive than MSCs isolated from adult tissue such as thebone marrow and adipose. Electromagnetic field (EMF) seems to be ableto change many biological functions such as proliferation, cell cycle,differentiation, DNA replication, cytokine expression, apoptosis, and etc.The aim of this study is to investigate the effects of electromagnetic fieldson the proliferation of WJ-MSCs.Methods: After isolating WJ-MSCs from umbilical cords Wharton’s jelly,the treatment, and control cell groups were cultured in DMEM medium.The treated cells were exposed to electromagnetic fields with a squarewave and 1mT intensity for 60 min exposure/15 min rest for 3 cycles at47 Hz for 3 days. The effects of EMF on cell viability and proliferation ofWJ-MSCs were investigated using MTT assay and trypan blue staining.Results: WJ-MSCs were significantly dead in the treatment group, whilein the control group, the cells proliferated and doubled after 3 days.Conclusion: While several studies have reported the effect of EMF onMSCs proliferation, but many of them have not mentioned to kind ofwaves they have applied. In our study, we used a square wave with 3cycles of 60 minutes, and we observed that this condition not only didnot affect the proliferation and survival of the MSCs but also leads tothe death of cells. It seems that this EMF condition may be effective indestroying and killing cancerous cells and be a good option for cancertherapy.

کلیدواژه ها:

Wharton’s jelly-derived mesenchymal stem cells ، Electromagnetic field ، Proliferation