Neural Differentiation of Human Induced Pluripotent Stem Cells on Polycaprolacton/Gelatin/Polyaniline Bi-electrospun Nanofibers

Background and Aim: In recent years, tissue engineering has gainedmuch attention because of its application in tissue and cell regeneration. Thus, in the past years, a lot of efforts have been given to improve tissueregeneration approaches. Recent advances in cell culture have resultedin the discovery of induced pluripotent stem cells. These cells appear toprovide great potential in comparison with any other types of stem cells,and thus, are more achievable and usable in terms of tissue regeneration.Nanofibrous scaffolds are considered as useful tools, in part becauseof their exceptional structure and potentials. Despite such efforts andachievements, the establishment of scaffolds with great features seem toneed greater efforts. Many polymers have been used for such purposes asa single form or in combination with other polymers.Methods: In this study, polycaprolactone (PCL) and gelatin (Gel) andpolyaniline (PANi) were exploited to engineer a composite for thedifferentiation of human induced pluripotent stem cells (hiPSCs) toneural cells. The composite was fabricated by electrospinning. MTT assaywas used to evaluate the toxicity on the composite. The hiPSCs wereseeded on the fibers and after 14 days of the differentiation, real-timePCR and immunocytochemistry (ICC) tests were performed to validatethe differentiation of the cells. For the analysis of morphological changes,scanning electron microscopy (SEM) was used.Results: Our findings showed a total differentiated of hiPSCs to neuralcells. This study highlights that PCL-GEL-PANi composite not only hasthe capability to support the differentiation of hiPSCs to the neural cellsbut also can improve the differentiation process. Taken all, PCL-GELPANiappears to be a feasible, reliable and simply accessed compositefor the tissue engineering approaches.Conclusion: Our results strongly confirm the potential of composites intissue engineering as they are shown and proven to support and enhancecell attachment, proliferation, and differentiation. It should be noted thatthe effect of PCL-GEL-PANi composite on the differentiation and cellsupport was significant.

کلیدواژه ها:

Human induced pluripotent stem cells ، Neural cells ، Tissue engineering ، Scaffold ، Polycaprolactone ، Gelatin ، Polyaniline