Mesenchymal Stromal Cell Labeling by NH2- Super Paramagnetic Iron Oxide Nanoparticles
- سال انتشار: 1397
- محل انتشار: سومین جشنواره ملی و کنگره بین المللی علوم و فناوری های سلول های بنیادی و پزشکی بازساختی
- کد COI اختصاصی: NSCMRMED03_185
- زبان مقاله: انگلیسی
- تعداد مشاهده: 427
نویسندگان
Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran
Cancer Research Centre, Tehran University of Medical Sciences, Tehran, Iran
Cancer Research Centre, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background and Aim: Bone marrow mesenchymal stromal cells (MSC)are multipotent cells and their ability to circulate and homing in differenttissue types as well as the lack of HLAII makes them a hot topic in celltherapies and regenerative medicine. However, it is necessary to makean optimized in vivo MSC tracking method. Superparamagnetic ironoxides (SPIOs) are a good candidate for a non-invasive efficient labelingmethod. The aim of this study is the development and evaluation ofan NH2- dextran-coated SPIO (NSPIOs) and optimization an efficientprotocol to label human MSCs (hMSCs).Methods: NH2-Dextran SPIOs were synthesized; Atomic ForceMicroscopy (AFM), Transmission Electron Microscopy (TEM), dynamiclight scattering and Zeta potential were used to evaluate the propertiesof the dedicated nanoparticle. Then, a new protocol optimized byusing heparin and protamine sulfate. The efficiency of iron content perhMSCs and viability of treated hMSCs compared to non-treated hMSCswere measured by inductively coupled plasma (ICP) and MTT assayrespectively.Results: Synthetized NSPIOs size constantly ranges around 70-90 nmbased on AFM, TEM and dynamic light scattering which increased to680 nm after heparin- protamine sulfate coating. After NSPIO-heparinprotaminesulfate treatment of hMSCs, 17± 0.39 pg per cell iron contentwas measured by ICP in compare to non-treated hMS cells and MTTassay did not show any effect of cells after NSPIO-heparin-protaminesulfate treatment.Conclusion: Our results show an efficient established protocol fortracking hMSCs. This tool can be used in tracing cells after transplantation.کلیدواژه ها
Human bone marrow cells; SPIO cell trackingمقالات مرتبط جدید
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