The Effect of Targeting EGFR Pathway in Glioblastoma Multiform

Background and Aim: Glioblastoma multiforme is an invasive type ofbrain tumor that penetrates a distinct attack from single glioblastomacells, which causes brain parenchymal infiltration. Here, we examinedthe effect of on miR-491 glioma cell line.Methods: Expression of miR-491 in glioma cells was assessed byquantitative PCR (qPCR). To assess the function of candidate microRNAs.In GBM, we transfected miR-491 to cultured-U-251 cell lines. Cellularproliferation an apoptosis was assessed by flow cytometry, and geneexpression in EGFR pathway was sassed by qPCR.Results: Our studies revealed that overexpression levels of microRNA-491can increase apoptosis and reduce cell proliferation in u-251 cell line.In addition, suppress the expression of epidermal growth factor receptor(EGFR) pathway.Conclusion: Taken together, these results revealed that overexpression ofmiR-491 in u-251 cell line might suppress the EGFR pathway.

کلیدواژه ها:

Glioma ، miR-491 ، Epidermal growth factor receptor