Study of the Eeffects of EMT Inhibitors on Cancer Stem-Like Cell (CSC) Enriched HT29 Cell Line Order to Anti-CSCs Drug Screening

Background and Aim: CSCs have a potential for self-renewal anddifferentiation and are resistant to current anticancer treatments. SinceCSCs generally comprise only small minorities within cancer cellpopulations screening for agents that specifically kill them may help toreduce cancer mortality. Studies showed that the EMT generates cellswith properties of CSCs. So, recently it has been noted to EMT inhibitorsas a promising strategy for CSC targeting. The aim of our research is thestudy of EMT inhibitors on CSCs enriched HT29 cell line in order to anti-CSC drug screening.Methods: Pioglitazone (Pio) was selected as an EMT inhibitor. CSCsenriched HT29 cell line by EMT induction using knockdown of E-cadherin,was cultured in DMEM media. MTT test was done in 3, 5, 7 days for IC50value calculating. After IC50 calculating, CSCs enriched HT29 cell linewas treated by the appropriate concentration of Pio. After drug treatment,properties and markers of CSCs and EMT were investigated compared tocontrol cells using several techniques including flow cytometry, RealtimePCR, and MTT assay. During the study, morphology changes weremonitored by optical and fluorescent microscope daily. The parentalHT29 cell line was used as the control.Results: Pio treatment of CSCs enriched-HT29 Cell line was causedby EMT inhibition. Cell morphology changed from mesenchymal toepithelial. Markers of CSCs and EMT were decreased compared tocontrol cells. Properties of CSCs and EMT were changed compared tocontrol cells.Conclusion: Pio as an EMT inhibitor can decrease CSCs in cancer cell line population. We expect significant advances in inhibition of EMTcan be useful for CSCs targeting. We suggest more study for the greaterunderstanding of EMT and CSC and their relationship to cancer treatment.

کلیدواژه ها:

Colon cancer ، Cancer stem cell ، EMT ، Anti CSCs drugs