Evaluation of Cord Blood Platelet Gel for The Treatment of Skin Lesions in Children with Dystrophic Epidermolysis Bullosa: A Pilot Study

Background and Aim: Epidermolysis bullosa (EB) consists of a number ofgenetically skin diseases characterized by defects in the adhesion of theepidermis to the dermis that cause mechanical fragility of the skin andabnormal wound healing. Wound care is, therefore, of critical importancein the management of EB and current cure are not always satisfactory. Inthis study, we performed a pilot evaluation of the effectiveness of plateletgel from cord blood (CBPG) as an allogeneic product for the treatment ofskin lesions in three children with EBMethods: Cord blood samples are collected in sterile conditions. These samples were transferred to GMP grade clean room for processing.All CB samples were negative for viral and bacterial infection. Then,bags contain CB were centrifuged and two parts (RBC and PRP) wereseparated. Gelation was done under sterile condition by adding thrombinwithin 20-30 minutes. Prepared gel changed twice per week, time andsize of wounds were measured.Results: The clinical outcomes of this small group of patients were shownthis trial have high efficiency and effectiveness in comparison with thecurrent standard of care for these patients. Time of healing and sizeof wounds decrease significantly. CBPG releases a number of growthfactors such as PDGF, TGF-β1 and -β2, IGF, EGF, FGF and EGF whichcan affect wound healing.Conclusion: In conclusion, this pilot study reveals that CPBG is apromising and safe option for the treatment of EB skin lesions but needfor larger studies.

کلیدواژه ها:

Epidermolysis Bullosa ، Cord blood ، Platelet gel