Effects of Astaxanthin on the Expression of PPARα and PPARγ in LPS-Stimulated Neuroblastoma Cells
- سال انتشار: 1397
- محل انتشار: سومین جشنواره ملی و کنگره بین المللی علوم و فناوری های سلول های بنیادی و پزشکی بازساختی
- کد COI اختصاصی: NSCMRMED03_119
- زبان مقاله: انگلیسی
- تعداد مشاهده: 425
نویسندگان
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background and Aim: Peroxisome proliferator-activated receptors(PPARα and PPARγ) activation can result in the transcription of proteinsinvolved in oxidative stress defense and mitochondrial biogenesiswhich could rescue mitochondrial dysfunction in Parkinson s disease(PD). Astaxanthin (ASTA) is a red-orange carotenoid with powerfulantioxidant that occurs naturally in a wide variety of living organisms.There is evidence demonstrating that ASTA confers neuroprotectiveeffects in experimental models of chronic neurodegenerative disordersand neurological diseases.Methods: In this study, Groups included the cell control group (SH-SY5Ycell line) that did not contain ASTA and/or LPS, the group that receivedthe ASTA (10 mM) alone, the group that received the LPS (10 mg/mL)alone and cell group was pre-treated with ASTA (10 mM) for 1 hour,washed, and then treated with LPS for an additional 24 h (LPS+ASTAgroup). ASTA was treated with, SH-SY5Y cell line at 48 hours. To measurethe expression of peroxisome proliferator-activated receptors (PPARα andPPARγ) and one of the genes involved in inflammation (IL-6). Using thereal-time PCR technique, the gene expression of PPARα and PPARγ weremeasured. Also, IL-6 protein expression was assessed by ELISA test. Theresults were analyzed by the one-way analysis of variance (ANOVA)using Prism version 6.0 software.Results: Our results show that treatment with ASTA (10 mM) for 1 hourattenuates the LPS-induced inflammation. The beneficial effect of ASTAis associated with the reduction inflammation by restoring the PPARproteins expression. ASTA has a greater effect on PPARγ expressionchanges than the PPARα.Conclusion: Consequently, accordingly hypothesize that ASTA hastherapeutic properties protecting SH-SY5Y cells from LPS-inducedinflammatory. PPAR proteins are very important in neuronal survival andinflammation. The results suggest that ASTA treatment may be beneficialfor enhancing oxidative stress defense.کلیدواژه ها
Astaxanthin; Neuroblastoma cells; Gene expression; PPARα; PPARγمقالات مرتبط جدید
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