Different Cytokines Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells
- سال انتشار: 1397
- محل انتشار: سومین جشنواره ملی و کنگره بین المللی علوم و فناوری های سلول های بنیادی و پزشکی بازساختی
- کد COI اختصاصی: NSCMRMED03_103
- زبان مقاله: انگلیسی
- تعداد مشاهده: 379
نویسندگان
Dept Oral Cell Biology, ACTA University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Dept Oral Cell Biology, ACTA University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Dept Oral Cell Biology, ACTA University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Dept Dental Materials Science, ACTA University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
چکیده
Background and Aim: During the initial stages of bone repair, somebiological phenomena emerge. Of these, proinflammatory cytokinesare released in the injury site, which quickly followed by a shift to antiinflammatorycytokines. However, the biological impacts of pro- and antiinflammatorycytokines on osteogenic differentiation of mesenchymalstem cells seems to be controversial. In this current investigation, westudied the effect of the proinflammatory cytokines (i.e., TNF-α, IL-6, IL-8,and IL-17F and the anti-inflammatory cytokine IL-4) on the proliferationand osteogenic differentiation of the human adipose stem cells (hASCs). Methods: In practice, the hASCs were treated with TNF-α, IL-6, IL-8, IL-17F, or IL-4 (10 ng/mL) for 72 hours mimicking bone repair.Results: Our findings revealed that the TNF-α could reduce the geneexpression of collagen type I, while it increased the proliferation ofhASC and the activity of ALP. IL-6 was found to significantly enhancethe activity of ALP (18-fold), while the bone nodule formation was alsoincreased by the hASCs. IL-8 did not affect the proliferation or osteogenicgene expression, however it lessened the bone nodule formation. IL-17Fwas found to decrease the proliferation of hASC, while it increased theactivity of ALP. IL-4 appeared to enhance the osteocalcin gene expressionand the activity of ALP; nonetheless, it decreased the gene expression ofRUNX2 and the bone nodule formation.Conclusion: Take all, our results showed that all cytokines studieddisplayed both inreasing and decreasing impacts on the osteogenicdifferentiation of hASCs, even when applied for 72 hours only. Somecytokines (i.e., IL-6) might be appropriate agents for the induction ofthe osteogenic differentiation of mesenchymal stem cells as a suitablestrategy for enhancing bone repair.کلیدواژه ها
Cytokines Osteogenic; Differentiation; Human adipose stem cells; Bone repairمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.