Survey of the prevalence of β lactamase CTX-M gene in isolates of urinary tract infections in patients by molecular method

Background: The Escherichia coli (E. coli) is one of the most common enterobacteriaceae causing Urinary tract infection (UTI). UTI is one of the most common bacterial infection and the section common infection which causes people to seek medical help. . On the other hand, UTI causes by E. coli producing extended spectrum beta-lactamases (ESBLs) is increasing worldwide. The aim of this study was to detect the prevalence of antibiotic resistance and CTX-M gene. Methods: During 6 months (June to November 2015) 266 clinical isolates of E.coli were collected from laboratories in Bonab. Phenotypic screening and confirmation tests for extended spectrum beta lactamases (ESBLs) were carried out using disk diffusion (Kirby Bauer) method. All of the ESBL producing isolates were tested by PCR using specific primers.10 PCR products randomly selected and purified and subsequently sent for sequencing. Results: As results showed, the maximum resistance was seen for ampicillin (67.3 %) and the maximum sensitivity was seen for imipenem (92.5%). In this study 45 % isolates were multidrug resistance, which showed at least resistance for three antibiotics. Out of 154 isolates, 58 (37.7%) isolates were ESBL producers which 94.8% of isolates contained CTX-M gene. Conclusion: The result of this study showed a high prevalence of CTX-M producing E. coli in Bonab. This poses a public hazard and grave concern, and it is necessary to take swift and appropriate measures to prevent it

کلیدواژه ها:

Escherichia coli ، UTI ، ESBLs ، CTX-M ، Bonab