CHROMagar™ MRSAversusCefoxitin for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods including Chromagar and cefoxitin disk diffusion with PCR based method as a gold standard for detection of mecA gene. Methods: A total of 220 clinical isolates of S. aureus which were isolated from various clinical specimens from September 2015 until the June of 2016 in Milad Hospital of Tehran, Iran was subject of our study. Methicillin resistance was determined by oxacillin and cefoxitin disks, oxacillin screen agar and CHROMagar™ MRSA medium. The results of these methods were compared with mecA gene based PCR method as a gold standard method. Results: Among 220 isolates from S. aureus, 105 (47.72%) isolates were positive for mecA gene by PCR method. The results of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method. CHROMagar™ MRSA medium had also 100 % sensitivity and 100% specificity. Oxacillin disk diffusion and oxacillin screen agar had 95.42% and 97.22% sensitivity respectively. Conclusion: Result of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method for detection mecA gene. The results of CHROMagar™ MRSA medium were comparable with PCR and cefoxitin disk diffusion methods.

کلیدواژه ها:

MRSA ، mecA gene ، CHOROM Agar ، PCR ، cefoxitin disk diffusion method