Campylobacter concisus and its effect on the expression of CDX1 and COX2

Background: Barrett’s oesophagus (BE) is a pre-malignant condition in which normal squamous epithelium of the lower oesophagus and gastroesophageal junction is replaced by columnar cells and progress to oesophageal adenocarcinoma. The increase burden of oesophagus cancer morbidity and mortality worldwide make study of factors involved in the pathogenesis of BE essential. However, most of studies that examine the environmental risk factors associated with increased incidence and prevalence of BE have largely ignored the potential role of bacteria in disease aetiology. This study examined the role of Campylobacter concisus isolated from Barrett’s and adenocarcinoma patient samples as one of possible environmental factors in the progression of Barrett’s oesophagus to oesophagus adenocarcinoma. Methods: We focused on the effect of C. concisus on the expression caudal type homeobox 1 gene (CDX1) and cyclooxygenase-2 (COX-2) in three BE cell lines using quantitative realtimePCR. In addition, theattachment and invasion characteristicsof C. concisus were also tested. Results: Results showed that C. concisus had a strong attachment to the cell lines and induce the expression of CDX1 in Barrett’s cell lines in a time-dependent manner. Conclusion: Findings indicate that C. concisus could be as a new challenge in the progression of BE to adenocarcinoma.

کلیدواژه ها:

Campylobacter concisus ، Barrett’s oesophagus ، COX2 ، CDX1