Determination of Acinetobacter baumannii Producing carbapenemase Isolated from Clinical Specimens by Hodge phenotypic Test Method in Sari, Iran

Introduction: The resistance of Acinetobacter baumannii strains to antibiotics is an important challenge for human health. The inappropriate use of antibiotics has led to antibiotic in microorganisms of Non fermentative family, especially in carbapenems. The research aimed to determine the prevalence rate of carbapenemases in Acinetobacter baumannii and antibiotic resistance patterns.Materials and Methods: In this cross sectional study, 100 Acintobacters were collected during a 6 month period, tertiary Hospitals of sari in 2017. Then, isolated Acinetobacters Were proved and identified using biochemical test, again. The antibiotic susceptibility test was performed by disk diffusion method. Also using Hodge phenotypic test and according Clinical & laboratory Standards Institute (CLSI) guidelines, carbapenemase enzyme producer isolates were isolated and identified.Ttest and spss 20 software were used for data analysis.Results: The results of antimicrobial sensitivity tests revealed that the highest resistance was against piperacilin (99%) Imipenem and cefotaxime (98%), whereas the highest susceptibility was observed for Gentamycin (14%).and 65 Acinetobacters isolates were carbapenemase enzyme producer.Conclusion: In the Present study, A. baumannii isolates in sari educational hospitals showed high resistance to different antibiotics. This study revealed that Acinetobacter baumannii isolated from patients were antibiotic resistant and carbapenemase producers.therefore, it is necessary to implement some approaches for the prevention of bacterial spread.

کلیدواژه ها:

Acinetobacter baumannii ، Antimicrobial Resistance ، Carbapenemase ، Hodge Test