Investigation of SNPs of CXCL8 and KLRG1 in PBMC OF SLE patients

Background: Systemic lupus erythematosus (SLE), also known as lupus, is an autoimmune disease in which the body’s immune system mistakenly attacks healthy tissue. Common symptoms include painful and swollen joints, swollen lymph nodes and a red rash on the face. The cause is not entirely clear. It is believed to involve hormonal, environmental, and genetic factors but genetic factors and SNPs play critical role.Material and method: Expression profile by array of PBMC of SLE patients were downloaded from GEO website. SLE patient and control were compared with logfc for level of gene expression. Genes were divided into hyperexpression in SLE and hypoexpression in control and vice versa. Then genes with smallest p-value in each group were chosen. All SNPs and variations of genes were identified with UCSC and NCBI webservers. SNPs that related to south Asia were extracted with ExAC. Inaddition, Pathways that related to these genes were identified with reactome and genecard webservers and drugs related to SNPs for these genes were identified.Results: CXCL8 was highly expressed and KLRG1 was down expressed in SLE patients with smallest p-value. Then some SNPs of these genes that are common in south asia was identified. Most important pathway of KLRG1 was adaptive immune system and it interact with PTPN11 and LYN. Also important pathway of CXCL8 was TLR signaling, this pathway interacts with RELA and CXCR4. Furthermore, some drug such as Simvastatin and ABT was identified that target CXCL8.Conclusion: Genes and their SNPs are associated with several diseases such as SLE. SNPs can affect protein structure and pathway, but ethnic variations of polymorphisms have very important role in seceptibility of variants for diseases So it is important to detect critical genes and their SNPs in each disease to help drug designing also it can help in personal medicine.

کلیدواژه ها:

Genes ، SNP ، Pathway ، Drug