The effect of pre-treated with nanoparticles of iron oxide (Fe2O3) to reduce the level of NF-kB gene expression in rats model brain Ischemia-reperfusion

Studies the role of NF-kB signaling pathway in the development of nerve cell death caused by ischemiareperfusion, as well as the development of inflammation in nerve tissue after a brain injury is. In thisregard, the nanoparticles of iron oxide (Fe2O3) protect neurons in the central nervous tissue againstoxidative stress has been proved. The aim of this study was to investigate the effect of pre-treatment ofFe2O3 nanoparticles on NF-kB gene expression is reduced.During the study, 40 Wistar albino rats were randomly divided into four groups, control grouppretreated with doses mg / kg 5 and mg / kg 10 respectively. Animals pre-treated and control groupsunderwent common carotid artery occlusion for 20 minutes with cerebral ischemia and reperfusion wasestablished by re-opening the arteries. were treated only by saline. 48 hours after induction of ischemia- reperfusion in brain tissue, animal euthanasia and animal brain hippocampus tissue was extracted andthe expression of NF-kB was evaluated using the Real time- PCR techniques.The results of the study showed was that relative gene expression NF-kB in the context of thehippocampus of animals in both dose 5 mg / kg and 10 mg / kg significantly compared to the controlgroup decreased (P-V doses of relationship (P-V <0.05).The results of the survey demonstrate the genomics was that pretreatment of Fe2O3 nanoparticlessignificantly to the performance factor NF-κB as a key factor NF-κB signaling pathway involved incontrolling brain tissue of mice ischemia-reperfusion Injury been brain

کلیدواژه ها:

Pretreatment ، iron oxide nanoparticles ، NF-kB ، cerebral ischemia reperfusion ، rats