Evaluation of Aflibercept effect on VEGF gene expression in CAFs and CAFs/epithelial cells of Oral Squamous Cell Carcinoma

  • سال انتشار: 1395
  • محل انتشار: اولین کنگره پزشکی شخصی
  • کد COI اختصاصی: IPMCMED01_127
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 523
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نویسندگان

Masoumeh Mirkeshavarz

Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences

Ahmad Nasimian

Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR

Pouyan Aminishakib

Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences

Meysam Ganjibakhsh

Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR

چکیده

Introduction: Oral cancer is the tenth most common cancer in Iran. The main reason of poor prognosis is early, distant metastasis. Recent investigations show tumor stroma and especially Cancer-Associated Fibroblasts (CAFs) have a considerable role in angiogenesis and consequently tumor progression. In the present study we investigated and compared the anti-angiogenic impact of Aflibercept (Anti-cancer drug) in both CAFs and CAFs/epithelial cells co-culture.Materials and Methods: After obtaining tissue samples from an oral cancer patient, CAFs and CAFs/epithelial cells were isolated using enzymatic digestion and explant culture. VEGF gene expression were investigated using Real-Time PCR before and after treatment by Aflibercept.Results: VEGF gene expression was significantly increased in CAFs/epithelial cells co-culture comparing with CAFs. After treatment by Aflibercept, although VEGF gene expression was decreased, it was not significant.Conclusion: Although Aflibercept may confine oral squamous cell carcinoma progression through decreasing VEGF gene expression, it seems other molecular factors interfere this process.

کلیدواژه ها

Oral Squamous Cell Carcinoma; Aflibercept; Carcinoma Associated Fibroblast

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