Toll-like receptors 4 cell membrane level in T lymphocytes of patients undergoing percutaneous coronary intervention (PCI)

Introduction: Atherosclerosis, the leading cause of cardiovascular disease (CVD), is driven by inflammation. Increasing evidence suggests that toll-like receptors (TLR) are key orchestrators of the atherosclerotic disease process. Toll-like receptor 4 is expressed by different cell types in the atherosclerotic vessel wall. Activation of TLR4 in atherosclerotic lesions stimulates mononuclear phagocytes to secrete chemokines which are recognized for their involvement in the recruitment of monocytes and T- lymphocytes in the arterial wall. Few studies have been done on TLR4 and its roles in T- lymphocytes recruitment and subsequent inflammatory response and hence, atherogenesis. Hence in this study the expression level of TLR4 in CD3+ T-lymphocytes was evaluated in Coronary artery disease (CAD).Material and methods: 45-65 years old patients that participated in this case-control study, were referred to shariati hospital for diagnostic angiography coronary for 13 months. Patients were derived into two categories, normal corner (n=38) and 3VD (n=38). Clinical information was recorded and angiography was performed, then patients CD were read by the intervention cardiologist. SYNTAX score for each patient was calculated. Cell surface markers, TLR4 were investigated by the flowcytometry in CD3+ T-lymphocytes.Results: T-test analysis showed that the means of the cell surface markers TLR4 (P=0.027) were enhanced in patients with coronary artery disease compared to the control group in coronary artery disease patients. Pearson test of synthax score and TLR4 level in CD3+ T-lymphocytes show significant (P= 0.04) positive correlation (0.224).Conclusion: Our findings illustrate that TLR4 is a risk marker against coronary artery disease. Synthax score, which grades the complexity of coronary artery disease, have positive correlation with TLR4 level. This result demonstrates TLR4 level augmentation on CD3+ T-lymphocytes is a marker of CAD severity.

کلیدواژه ها:

Coronary artery disease (CAD) ، Toll Like Receptor4 (TLR4) ، SYNTAX score