Effects of Low Level Laser Photobiomodulation and P-coumaric Acid on Lactate Dehydrogenase Activity of Melanoma Cancer Cells

The cancer cells demonstrate high rate of glycolysis instead of oxidative phosphorylation, to generate energy for rapidly proliferating tumor cells. The lactate dehydrogenase (LDH) enzyme catalyzes the reversible transformation of pyruvate to lactate under anaerobic conditions, coupled with the oxidation of NADH to NAD+. Numerus studies have suggested that elevated LDH is hallmark of aggressive form of carcinomas. In this study, LDH activity was determined in human melanoma A375 cancer cells treated with low level laser (LLL) photobiomodulation and then p-coumaric acid. Human melanoma cancer cell line A375 was exposed to irradiation with a red laser source (660 nm; power density: 30 mW cm-2) and another sample was kept in dark as control. After treatment with laser, the cells were treated with different concentrations of P-coumaric acid (0-1 mg/mL) for overnight. The LDH assay was used to determine the enzyme activity. The morphology of cells was studied using inverted light microscopy. The results indicate that early treatment with low level laser photobiomodulation and then P-coumaric acid decreases the LDH activity as compared with the dark group. As LDH is a promising target for anticancer therapy, application of low level laser photobiomodulation and then P-coumaric acid could be considered as novel opportunities in human melanoma cancer treatment.

کلیدواژه ها:

Human Melanoma Skin Cancer ، P-coumaric Acid ، Photobiomodulation (PBM) ، Lactate Dehydrogenase (LDH)