Chitosan / Modified Egg Shell Wound Healing Scaffold Containing Vitamin D and Herbal Remedy for Diabetic Wounds

Aim and Background: Freeze Drying is a modern method for high level of stability and long time maintain in the field of medicine and physician. One of the areas where the use of this method is very impressive, it is the synthesis of wound healing scaffolds that can be accompanied by various repair and excitation factors for wound healing. In this article, the use of a restorative plant extract, egg shell as an agent containing calcium phosphate and collagen that has antibacterial properties and also silver nanoparticles is emphasized. Method: In the preparation method, scaffolds for wound healing was prepared by freeze drying of a solution containing sterilized and powdered egg shells, almond and Mahaleb extract, silver nanoparticles, and vitamin D. Each scaffold had certain contents and the chitosan were used as the main material for the construction of scaffolding in all scaffolds. Each solution of Chitosan, AgNps, vitamin D, herbal extracts and egg shells were dissolved in acetic acid, phosphoric acid and water soluble solvents, and various ratios were prepared and put on special plates for freeze-drying. Afterwards The work and preparation of scaffolds were used for various antibacterial tests and etc. tests. Results and discussion: The Scanning Electron Microscope images of the scaffolds proved that porous structures are formed in the form of interconnected which can facilitate the exchange of air and oxygen, as well as the materials needed by the cells in the wound site. Presence of chitosan, AgNps and egg sell in the scaffold has led to the creation of an appropriate antibacterial property in the structure and resists against bacteria, which are mainly gram-positive staphylococcus aureus which these findings are derived from antibacterial test of nanoparticles, scaffolds, and also Minimum Inhibitory Concentration test. Conclusion: The results of this work showed that egg sell incorporated AgNps and chitosan have a great potential to be used as the biodegradable, bio-based and antibacterial wound healing scaffold.

کلیدواژه ها:

Chitosan ، Ag Nanoparticles ، Scaffold ، Staphylococcus Aureus ، Vitamin D