A glassy carbon electrode modified with a new simple nanocomposite film basedon multiwall carbon nanotubes and zein nanoparticle for simultaneousdetermination of ascorbic acid, dopamine and uric acid

Zein is the major storage protein in corn or maize, has attracted attention because of its potentialapplications as an industrial biopolymer. Zein protein can form composites or conjugates with othercompounds such as nanomaterials and also in encapsulation processes. Therefore, in recent years,several research groups have reported the use of nanoparticles in different research areas such asdrug delivery, food science, pharmaceutics, and biomedicine [1]. Carbon nanotubes (CNTs) havebeen considered as an important class of nanomaterials with excellent mechanical, electrical andchemical properties since they have been discovered. These unique properties make themextremely attractive for fabricating electrochemical sensors because of the ability of CNTs tofacilitatethe electron transfer reactions [2]. However, no works have been reported about the use ofzein/multiwall carbon nanotubes nanocomposite for glassy carbon electrode modification forelectroanalytical applications. For a mixture containing ascorbic acid (AA), dopamine (DA) anduric acid (UA), the three compounds can well separate from each other at the scan rate of 100 mVs−1 with a potential difference of 250 mV and 160 mV for the oxidation peak potentials of AA–DAand DA–UA, respectively, which was large enough to simultaneous determine AA, DA and UA.The catalytic peak current linearly depended on the AA, DA and UA concentrations in the range of8.55–812 μM, 7.85–824 μM and 0.33–73.3 μM, respectively. The detection limits for AA, DA andUA were 2.11, 2.05 and 0.056, respectively. The analytical performance of this sensor has beenevaluated for simultaneous detection of AA, DA, and UA in human serum and urine samples.

کلیدواژه ها:

Multiwall carbon nanotubes ، Zein nanoparticle ، ، Ascorbic acid ، Dopamine ، Uric acid