The Effects of Combination Therapy of Caffeine and Glibenclamide on Catalase Enzyme of Renal Tissue in Diabetic Rats

Oxidative stress can lead to a variety of harmful effects on the performance of human internal organs, particularly its effect on human kidney as diabetic nephropathy as a serious complications. Such complications can destructively affect kidney’s glomeruli function. To alleviate kidney’s glomeruli mal-functionality due to diabetic nephropathy it is recommended for patients to intake glibenclamide as one of the widely prescribed medications. However, glibenclamide may show several adverse effects including hypoglycemic shock. To overcome the aforementioned adverse effect, it is suggested to use caffeine as a supplementary medication as an anti-oxidant in addition to protecting cells structure. In this study, the effect of combination therapy of caffeine and glibenclamide was assessed on catalase enzyme activity of renal tissue in diabetic rats. Materials and Methods:In this experiment, 32 male rats, artificially induced-diabetic by intraperitoneal injection of streptozotocin (Dosage: (60 mg/kg) weighting 200-250 g were considered. In the beginning of experiment, rats were randomly divided into four main groups as follows: Group 1- Diabetes mellitus (DM), Group 2- DM + glibenclamide, Group 3- DM + caffeine, and Group 4- DM + glibenclamide + caffeine combined. After three days post-injection, diabetes was verified using glucometer. The treated groups, i.e., Groups 2, 3 and 4, respectively received 0.3 mL of glibenclamide, 0.5 mL caffeine and 0.15 mL glibenclamide and 0.25 mL caffeine combined. In the second week after drug administration, rats were anesthetized, Then, removed kidneys of all rats were examined after homogenizing and centrifuging. Catalase enzyme activity was measured by Beer & Sizer method, and then evaluated by the Spectrophotometry. Quantitative data was analyzed by one-way ANOVA using SPSS. Results: Treatment of diabetic group with glibenclamide significantly enhanced catalase activity versus untreated diabetic rats (p<0.05) and caffeine treatment of diabetic group did not significantly change catalase activity. In addition, treatment of diabetic rats with a combination of glibenclamide and caffeine also significantly increased catalase activity versus diabetic one (p<0.05). Conclusion: It was found out that glibenclamide and combination of glibenclamide and caffeine could properly increase catalase activity in kidney tissue of diabetic rats and this may be helpful in delaying the progression of diabetic nephropathy.

کلیدواژه ها:

Caffeine ، Diabetes Mellitus ، Kidney ، Catalase ، Rat