Study of relationship between colorectal CD133 and CD24 cancer stem cells with clinicopathologic characteristics of colorectal cancer

Background: Cancer stem cells are group of tumor cells that have regeneration and multi-order differentiation capability. These cells were detected in colorectal cancer with expression of one or more cell surface markers in various studies such as CD24 and CD133. In order to study relationship between these markers and clinic-pathological characteristics and patients’ survival, we studied their expression in colorectal cancer and normal adjacent tissues. Material and Methods: Expression of CD24 and CD133 was studied in paraffin block of colorectal cancer and normal tissue near tumor with immunohistochemical method in patients referring to Imam Khomeini Hospital in Sari and the relationship between clinical and pathological characteristics and condition of patient regarding metastasis, recurrence and death caused by tumor was studied. Result: 50 samples (25 males and 25 females) with mean age 67.57±13.9 years old with range 28-93 years included 3 mucinous carcinoma and 74 adenocarcinoma.Expression of CD133 marker was negative in 29 cases and positive in 21 cases. Expression of CD24 in tissue near tumor was found in 30% of available samples (24 out of 30 cases). The relationship between expressing CD24 with treatment (surgery and chemotherapy) was significant and its relationship with patient’s survival was insignificant; however,there was clear difference (mean survival age for patients based on CD24 expression was 26.64±18.15 for negative cases and 41.75±28.76 months for positive cases). CD24 and CD133 expressions and their co-expression with other clinic-pathological factors were not significant. Conclusion: During this study, the relationship between CD24 with treatment type was significant. To confirm result, various studies with high numbers of samples and other stem cell markers are recommended.

کلیدواژه ها:

Cancer stem cells ، Colorectal cancer ، CD24 ، CD133 ، Clinicopathologiccharactristics ، Metastasis