The Effects of combination therapy of Caffeine and Glibenclamide on Oxidative Stress of Testis Tissue among Streptozotocin-Induced Diabetic Rats

سال انتشار: 1394
محل انتشار: هفدهمین همایش سالانه آسیب شناسی و طب آزمایشگاه
کد COI اختصاصی: ACPLMED17_012
زبان مقاله: انگلیسی
تعداد مشاهده: 422

نویسندگان

Student Research Committee, Faculty of Medicine, Shahed University, Tehran, IR Iran

Biochemistry Department, Faculty of medicine, Shahed University, Tehran, IR Iran

چکیده

Background and Objective: Diabetes has destructive effect on testis function because of oxidative stress and free radicals it has made. Glibenclamide is one of the material that is widely used in diabetes. Other material that is widely used in various countries is caffeine that has anti-oxidant effect. This study was conducted to survey the combination therapy effect of caffeine and glibenclamide on oxidative stress of testis tissue among diabetic rats. Materials and Methods: In this experimental investigation, 32 male rats, weighing 200 - 250 g, were divided into four groups: 1: diabetic control without treatment, 2: diabetic receiving treatment with glibenclamide, 3: diabetic receiving treatment with caffeine, and 4: diabetic receiving treatment with glibenclamide and caffeine. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). On the third day after the injection of streptozotocin to ensure the mice are diabetic, urinary glucose test strips was used. Treated group with glibenclamide get daily 0.3cc, treated group with caffeine get daily 0.5cc and the group treated with glibenclamide and caffeine get daily respectively, 0.15cc and 0.25cc by intraperitoneal injection. In the second week after drug administration, rats were anesthetized and by opening the peritoneal cavity their testis removed and after homogenizing and centrifuging, by using supernatant, MDA was measured by Thiobarbituric method and protein measurement was done by Bradford method and then it was evaluated by spectrophotometry. Quantitative data were analyzed using one-way ANOVA. Results: First we knew that all the rats are diabetic by urine test done, then on the third day with statistical analysis results showed that the use of glibenclamide or caffeine alone in diabetic rats doesn’t make a significant decrease in the testis tissue level of MDA. The same condition was seen about using caffeine and glibenclamide simultaneously. (p> 0.05) Conclusion: In short, it was found that using glibenclamide, caffeine or the combination doesn’t have a beneficial effect in reducing lipid peroxidation in testis of diabetic rats.

کلیدواژه ها

Diabetes Mellitus; testis; Oxidative Stress; MDA; Rat

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