First report of Clonal evolutionMultidrug-Resistant Acinetobacter baumannii in west of Iran by PFGE

Introduction and Objectives:A baumannii is usually multidrug resistant (MDR), including third generation cephalosporins,amino glycosides and fluoroquinolone. Resistance to these antibiotics is mediated by multiplefactors such as: lactamases, efflux pumps and other mechanisms of resistance. Pulsed-FieldGel Electrophoresis (PFGE) was then used to investigate the genetic relationships among themultidrug-resistant (MDR) isolates. The aim of this study was to determine MDR isolates andthe existence of OXAs genes among MDR isolates of A. baumannii collected fromKermanshah hospitals in west of Iran.Materials and Methods:Forty-two MDR A. baumannii were collected from patient at Kermanshah hospitals. Theisolates were identified by biochemical tests and API 20NE kit. The susceptibility to differentantibiotics by disk diffusion method (CLSI) was determined. PCR was performed fordetection of blaOXA-23-like, blaOXA-24-like, blaOXA-51-like and blaOXA-58-likebetalactamase genes in isolates and clonal relatedness was done by PFGE (with the restrictionenzyme ApaI) and Patterns analyzed by Bionumeric software.Results:This study showed high resistant to ciprofloxacin, piperacillin, ceftazidime and also resistantto other antimicrobial agent and more spread blaOXA-23-like gene (93%) in MDR isolate.The PFGE method obtained 6 clones: A (10), B (9), C (5), D (4), E (11) and F (3) that cloneE was outbreak and dominant in different wards of Hospitals studied. Most of isolates inclone A, B and E antibiotic resistance were higher than other clones.Conclusion:An isolate from the emergency ward of these hospitals had an indistinguishable isolatesPFGE profile and similar resistance profile to isolates from ICU, suggesting likelytransmission from ICU to emergency via patient or hospital staff contact. Only tigecyclineand colistin remains effective for the treatment of infections caused by MDR A. baumannii.

کلیدواژه ها:

Acinetobacter baumannii ، multidrug resistant ، OXA-type ، PFGE