Investigation of the Gasoil Inductive Effects on Blood Parameters of White Albino NMRI Mice

Background & Aims of the Study: Gasoil is one of most-used oil products applied asdiesel fuel, for instance, which, since it consists of aromatic compounds, is among the mostimportant environmental pollutants. Considering its harmful effects on living organismsand human beings, the purpose of the present study was to investigate the effects of gasoilingestion on blood factors of white Albino NMRI mice.Materials & Methods: 40 adult mice, with an average weight of 28 grams, were placed infour groups. Adult male mice were placed in two groups, including a control group and atreatment group and adult female mice were divided into two groups of control andtreatment. For 14 days, the treatment groups were fed once per day with 0.2cc of puregasoil solution with a ratio of 6.6 mg/kg of the mice’s weight. After anesthesia and bloodsample collection, blood parameters, including the number of white and red blood cells,hematocrit and hemoglobin were respectively measured using Neubauer slide, capillarytube, and Sally method. Then, the analyses performed using SPSS19 software.Results: Variations observed in the blood parameters of male and female mice placed inthe treatment groups compared with the control group indicated a significant increase inhematocrit (8%, 10%), a significant decrease in hemoglobin (6%, 10%), and no significantincrease in the average number of RBCs (6%, 6%). The level of blood leukocytesconsisting of lymphocytes and neutrophils indicated a decrease, while the level of blood.leukocytes consisting of degenerated lymphocytes indicated a significant increase (P<0.05).Conclusions: Due to the increased use of gasoil and generalization of the results of thepresent research to human beings in terms of leukocyte reduction and weakening of theimmune system, hemoglobin reduction and tissue oxygenation disorder, in addition toenvironmental damages, this substance imposes irrecoverable damages on human health.Hence, necessary measures should be taken by authorities for replacing, reducing its effectsand raising public awareness of the methods of dealing with its effects.

کلیدواژه ها:

Gasoil ، Blood Parameters ، White Albino NMRI Mice