THE EFFECT OF LACTOBACILLUS CASEI.ON SKIN WOUND HEALING IN WISTAR DIABETIC RATS BY GAVAGE METHOD ON DAYS 1,3,7,14,21

Background and Aim:Probiotics are microorganisms in the digestive tract (Gastrointestinal tract). In addition to helping the digestion of complex molecules, also it helps the immune system and reduces inflammation. The beneficial effects of this bacterium on gastrointestinal ulcer healing have been proven eventhough Quantitative research has been done on the therapeutic effects of these bacteria on skin ulcers, especially the repair of skin lesions in diabetic people. The aim of this study was to investigate the effect of Lactobacillus casei.on skin ulcer healing.Methods:After isolating the probiotic bacteria strains and examining the production of exopolysaccharides, Lactobacillus casei with the ability of producing exopolysaccharides, were selected. Then, Wistar male rats were divided into 2 groups: 1 group of 10 Control group and one experimental group of 15 experimental groups including gavage control, experimental gavage and then diabetic rats with diabetic streptozotocin, and a square scar in the size of 1.5 × 1.5 cm on mice back was made. . After 24 hours of wound healing, the experimental group of PBS gavage containing Lactobacillus casei but the control group did not receive any treatment. The scar area was measured every 3 days. The skin of the killed rats on days 1,3,7,14,21 were taken and under histological and statistical studiesResults:Thereafter, there was a progressed increase in ulcer healing on day 7 in animals treated with dead probiotics (p= 0.03) as compared to untreated controlsConclusion:probiotic bacteria consumed decreased inflammation

کلیدواژه ها:

Skin ulcer ، Diabetes ، Probiotics ، Lactobacillus casei ، Exopolysaccharide