GENOMIC DETECTION OF CHLAMYDOPHILA ABORTUS IN SHEEP VAGINAL SWAB SAMPLES WITH ABORTION HISTORY IN LORESTAN PROVINCE USING NESTED-PCR METHOD

Background and Aim:Chlamydophila abortus is one of the most important infectious agents, causing abortion in ruminants. C.abortus is responsible for ovine enzootic abortion in sheep and goats, but is less commonly in cattle. Chlamydial abortion typically occurs in the last 2-3 weeks of pregnancy with the appearance of stillborn lambs and inflamed placentas. In Lorestan Province due to lack of sufficient information about Chlamydia aborttions because of the mountainous region sheep breeding is very important and considering to economic losses of abortion finding out the factors is very important.Methods:In this cross – sectional study (from October 2017 – May 2018), 210 sheep vaginal swab sample were collected of aborted sheep in Lorestan Province. Samples were tested for the presence of Chlamydophila abortus by Nested PCR method related to 16srRNA gene.Results:In this study, 19 samples (9.04%) were found to be positive for the presence of Chlamydophila abortus.Conclusion:It can be concluded from this study that Chlamydophila abortus is one of infectious agents abortion in Lorestan Province and we have to do more studies and vaccination program can be used as a preventive method.

کلیدواژه ها:

Chlamydophila abortus ، Nested – PCR ، sheep vaginal swab ، Lorestan province