ANTIMYCOTIC EFFECT OF MENTHOL ON EXPRESSION OF SAP1-ENCODING GENE IN CANDIDA ALBICANS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 366

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شناسه ملی سند علمی:

MEDISM19_625

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Using of biological compounds such as menthol could be one of the most frequently ways to alleviate antifungal resistance phenomenon. Hence, in this study, we endeavored to investigate the effect of menthol on the morphology switching in Candida albicans due to expression of SAP1 gene.Methods:The relative minimum growth inhibitory concentrations were determined by broth microdilution according to the CLSI M27A3 standard protocol with slight modification for all isolates with both menthol and fluconazole. Eventually, the expression level of one of the effective genes in hyphae production of Candida albicans was measured using Real Time RT- PCR.Results:MICs for fluconazole were reported in all isolates that ranged from 1 to 64 μg/ml, while the menthol content of this ranged from 0.8 to 25 μg/ml, and all isolates showed a ratio of sensitivity. The result of the analysis of the expression of SAP1 gene involved in hyphae production for two concentrations of fluconazole equals 2MIC and MIC showed 1.85 and 1.86-fold reduction, respectively (P≤ 0.05) while, for menthol was decreased ranged from 2.02 to 1.85-fold, respectively in 2 MIC and MIC concentrations (P≤ 0.01).Conclusion:The results of this study showed that the secretion of aspartyl proteinase enzyme in the presence of hyphae could be effective in pathogenicity and deterioration of host tissues and is reduced in the presence of menthol compound. In addition, the Real Time RT-PCR analysis of SAP1 gene suggested the probable molecular targets of methanol in Candida albicans.

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نویسندگان

Shahrzad Shayegan

Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran

Alireza Khodavandi

Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran