STUDY PHYLOGENETIC GROUPS OF PATHOGENIC E.COLI STRAINS ISOLATED FROM PATIENTS IN IMAM REZA HOSPITAL, KERMANSHAH

Background and Aim:Escherichia coli strains have been assigned to four main groups – A, B1, B2 and D. Group A and B1 are generally associated with commensals, Strains from phylogenetic group B2 and D are generally more virulent, but also less resistant and contain resistance encoding integrons to a lesser extent than strains from groups A and B1. The aim of this study is to determine the prevalence rate of each phylogenetic group of E. coli strains obtained from urinary tract infections.Methods:96 clinical samples have collected from the patients referring to Imam Reza hospital in Kermanshah, Iran. The samples were cultured on MacConkey agar medium and subsequently various biochemical tests were performed to confirm the isolates. DNA extraction of E. coli isolates was done by boiling method. To classify the E. coli strains, specific primers for the chuA and yjaA genes and the TSPE4.C2 were applied for the PCR test.Results:Of all samples collected from patients, a total of 98 isolates were identified as E. coli by conventional biochemical methods. The PCR method showed that the most common phylogroup was group B1 (33 isolate; 33.37%), followed by groups B2 (24 isolate, 25%), D ( 22 isolate,22.91%) and group A(17 isolate, 17.7%).Conclusion:: Our study revealed the frequency distribution of phylogenetic groups in different strains of E.coli in our study has been as B1> B2> D> A. Further studies in addition to controlling the infections caused by E.coli contribute to the reduction of MDR strains.

کلیدواژه ها:

E.coli ، PCR ، chuA ، yjaA