PSEUDOMONAS AERUGINOSA VIRULENCE MARKERS

  • سال انتشار: 1397
  • محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
  • کد COI اختصاصی: MEDISM19_575
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 476
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نویسندگان

Shahrbanou Izadi Pour Jahromi

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

Jalal Mardaneh

Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

Asghar Sharifi

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

چکیده

Background and Aim:Pseudomonas aeruginosa is a gram negative, bacillary shape, non-spore forming, ubiquitous opportunistic bacterium present in many various environmental settings in different geographical regions, and it can be isolated from diverse living sources, such as humans, animals, and plants. The goals of the current multicenter research were to assess virulence factors genes among the P.aeruginosa clinical isolates.Methods:Pseudomonas aeruginosa isolates were recovered from inpatients. Bacterial identification was done by standard diagnostic tests, and species was confirmed by detection of exoA gene by PCR technique. Virulence markers were detected by PCR.Results:Among surveyed isolates, plcH+, lasB+ genotype (100% isolates) were the most common virulence genes patterns. Thirty-nine (48.75%) out of 80 P.aeruginosa isolates showed algD+, plcH+, lasB+, IMP+genotype. The blaIMP resistance gene was detected in all MHT positive and MDR isolates. Another interesting finding in our study was the more frequent occurrence of algD+, MDR, MHT+ and blaIMP+ strains among the Pseudomonas aeruginosa isolates from Yasuj compared with those from Shiraz.Conclusion:Emergence of multi-drug resistant P. aeruginosa, especially to carbapenems and aminoglycosides, and the high prevalence of virulence traits in our study could be regarded as an alarming situation.

کلیدواژه ها

Virulence Markers, Pseudomonas aeruginosa, Hospital

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