CELL EPITOPE-BASED VACCINE CANDIDATE AGAINST BACTEROIDES FRAGILIS BY IMMUNOINFORMATICS APPROACH
- سال انتشار: 1397
- محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM19_540
- زبان مقاله: انگلیسی
- تعداد مشاهده: 424
نویسندگان
Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
چکیده
Background and Aim:Bacteroides fragilis is one of the most abundant anaerobes found in humans and cause intra-abdominal infection, sepsis, soft tissue abscess and bacteraemia. The traditional approach to design a vaccine is a long process that involves the attenuation of the pathogen through sub-culturing followed by its administration. The administration of whole pathogen raises several safety issues and toxicity owing to the unwanted biomaterial. Thus, peptide can also serve as a vaccine candidate, as well as can be used for designing immunotherapeutic. It has been shown in the past that a small peptide or antigenic regions can also activate different arms of the immune system. The conventional approach for designing vaccine against a particular disease involves stimulation of the immune system using the whole pathogen responsible for disease.Methods:First, vaccine targets were selected according to standard characteristic. Then B-cell epitope of canditate peptide were predicted using well-known online bioinformatics server (IEDB, Bcepred, Ellipro, DiscoTope) and then were selected and compared based on the highest score and the highest repetition.Results:In this study, we designed a new structural model containing two putative antigenic determinants of SusC/RagA family TonB-linked outer membrane protein and membrane protein insertase YidC fused together by hydrophobic linkers.Conclusion:The fusion peptide construct may be used as epitope-based vaccine for development of Bacteroides fragilis vaccine candidates.کلیدواژه ها
Immunoinformatics; in silico; epitope based vaccine; Bacteroides fragilisاطلاعات بیشتر در مورد COI
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