ANTIBACTERIAL AND ANTICANCER ACTIVITY OF A BIOFLAVONOID FRACTIONATED FROM ALLIUM ASCALONICUM

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 489

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شناسه ملی سند علمی:

MEDISM19_453

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim: Shallots are an important part of the diet of many populations and there is long-held belief in their health enhancing properties. Shallot extract prevent the proliferation of cancer cells and has anti-bacterial properties. The objective of this study was the evaluation of antibacterial and anticancer activity bioflavonoid fractionated from Allium ascalonicum against some bacteria and cancer cells by MIC and MTT methods.Methods: The effect of cytotoxic extracts using MTT colorimetric assay was performed. Doxorubicin was used as a positive control sample and medium containing 5% DMSO as a negative control in cell-free extracts will be considered. Plates incubated for 48 hours in CO2 5% and then at 37 ° C. 100 ml solution of DMSO to dissolve the formazan crystals replace the previous solution and absorption at a wave length of 560 nm is read by Elisa reader. Also the effect of antibacterial extract was evaluated by MIC method.Results: Allium ascalonicum showed antifungal and anticancer activity. C.diphtheriae was most sensitive, P. aeruginosa and S. pyogens were most resistant bacteria tested. In our study the effect of shallot extract was a dose-dependent pattern and the highest sensitivity cells was observed after 72 hours.Conclusion: It is of interest that the extract of this plant has shown much less cytotoxicity against the normal cell line. The results of our study indicated that A. ascalonicum can be a candidate for treatment of many diseases.

نویسندگان

Razieh Aliyan

Department of Food and Drug Control, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Mansour Amin

Department of Microbiology, Infection and Tropical Diseases Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

Saeed Khoshnood

Department of Microbiology, Infection and Tropical Diseases Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran