ENTROCINS PRODUCED BY THE STRAIN ENTEROCOCCUS FAECALIS T23

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 327

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شناسه ملی سند علمی:

MEDISM19_451

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:We have partially characterized the bacteriocin activity of the strain Enterococcus faecalis T23. The antimicrobial activity of this strain was determined against Listeria monocytogenes, Salmonella typhimurium and closely related Lactobacilli strains. Treatment with different enzymes revealed that the active substance has a protein nature. PCR amplification resulted in detection of two bacteriocin genes:entB and entA. Bacteriocins of the studied strain were heat stable and active over a wide range of pH (3–10). Triton X-20,Triton X-80, Triton X-100, β-mercaptoetanol, Na-EDTA, SDS and NaCl did not influence the bacteriocin activity.Methods:Identification of bacteriocin producer strain investigated by phenotypic and genotypic 16srRNA.Heat and pH treatments and biochemicals were done.entrocin genes was performed by entrocin specific primers.Results:E.faecalis T23 presented antimicrobial activity against Listeria monocytogenes EGDe107776, Salmonella typhimurium and also closely related Lactobacilli strains, such as Lb.brevis F145, Lb. bulgaricus L340.PCR amplification with specific primers resulted in detection of two bacteriocin genes in E.faecalis T23: entB and entA. Both of identified enterocin genes encode bacteriocins, which belong to class II bacteriocins.Conclusion:In the present study it was shown, that strain E. faecalis T23 produce antimicrobial compound with strong anti-Listerial activity.These compounds are most probably bacteriocins, as they are heat-stable and sensitive to proteolytic enzymes

نویسندگان

Tannaz Mirhadizadi

Ferdowsi University Of Mashad