OPRM CLONING OF PSEUDOMONAS AERUGINOSA BACTERIA IN ESCHERICHIA COLI AFTER BIOINFORMATICS CONFIRMATION AS A CANDIDATE FOR VACCINE

Background and Aim:Pseudomonas aeruginosa is an opportunistic pathogen that infects immunosuppressed individuals and compromised tissues such as burn wounds, damaged corneal tissue, and the trachea of intubated patients or patients with cystic brosis. On the other hand, the resistance to antibiotic drugs for this bacteria is increasing. Perhaps, choosing the right vaccine can solve the problem. So, we suggest other membrane protein M (OprM).Methods:Bioinformatics studies were performed on Pseudomonas aeruginosa. Primer design, PCR (Polymerase Chain Reaction) and cloning for oprM in the plasmid pET 32a done. Then, its transfer to Escherichia coli BL21 were performed. Finally, it was approved by universal primer PCR and enzymatic digestion methods.Results:The bioinformatics studies shown high conservation and strong antigenic properties for OprM protein. In the laboratory experiments, the oprM was cloned in Escherichia coli BL21. And approval tests were positive.Conclusion:Cloning ability of this gene is suitable for the evaluation of the target vaccine.

کلیدواژه ها:

Pseudomonas aeruginosa ، oprM ، vaccine ، Clonning