ASSESSMENT THE PHYSICOCHEMICAL PROPERTIES, STRUCTURE AND FUNCTION OF FUNGAL STRAINS LIGNIN PEROXIDASE PHANEROCHAETE CHRYSOSPORIUM
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 352
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شناسه ملی سند علمی:
MEDISM19_292
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Phanerochaete chrysosporium is a fungal strain with high metabolism competence aromatic hydrocarbons based on synthesis and secretion of lignolitic enzymes including lignin proxidase . Lignin proxidase as glycoprotein enzyme with high redox potential and resistance in low pH. Therefore, identification the functional and structural properties of this enzyme and its isoforms in different strains is a crucial step in bioremediation of environmental pollutions or development new anti-cancer food drugs.Methods:Selective protein sequence with access number AF140062 was obtained from NCBI database and physicochemical properties of them were. The relationship among fungal and non-fungal strains were determined via MAGE.6 program through PAM250- BLOSUM62 matrices. Structural modeling of selected enzyme with homology method using SWISSMODEL and Modeler program, active site was determined after quality evaluation was performed RAMPAGE and MOE tests. thermal stability of enzyme was performed with a molecular dynamics simulation in industrial temperature using GROMACS test. Finally, functional capability of enzyme was determined by molecular docking test ratio aromatic hydrocarbons obtained from PUBCHEM database.Results:The results of this study led to the achievement of the structure of enzymes and homolog while it was good quality, binding affinity and different functionality they showed against aromatic hydrocarbons . So that, on average between 11 hydrocarbon selective binding affinity with Dibenzo (a, i) pyrene highest and the lowest was Anthracene.Conclusion:However, the ability favorable physico-chemical and thermal stability of the mother enzyme and structural optimization, synthesis and subsequent tests were on the agenda.
کلیدواژه ها:
نویسندگان
Nasrin Sanayipoor
Department of Biology, Islamic Azad University, KhorasanRazavi, Neyshabur, Iran
Aliakbar Haddad-Mashadieizeh
Department of Cellular and Molecular Biology, Faculty of Science and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
Samaneh Dolatabadi
Department of Biology, Islamic Azad University, KhorasanRazavi, Neyshabur, Iran