GENETIC ANALYSIS OF NONRIBOSOMAL PEPTIDE SYNTHESIS GENES (NRPSS) IN THE FRESH WATER CYANOBACTERIAL OF THE LAVASAN LAKE
- سال انتشار: 1397
- محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM19_277
- زبان مقاله: انگلیسی
- تعداد مشاهده: 483
نویسندگان
Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
چکیده
Background and Aim:Among the photosynthetic microorganisms, cyanobacteria are regarded as good candidates for drug discovery, with applications in agriculture, industry and especially, in pharmaceuticals. To date, the majority of bioactive metabolites isolated from cyanobacteria have either been polyketides, non-ribosomal peptides, or a hybrid of the two. Methods:In this study, efforts have been undertaken to investigate of ten cultured fresh water cyanobacteria strains of the Lavasan Lake. These strains were identified based on the sequence of 16 SrRNA gene and found that these strains belong to the Nostocaceae, Scytonemataceae and Rivulariaceae. The antibiogram bioasyas were screened in phototrophic condition using a paper disc method. The results showed that maximum antifungal activity was seen in Nostoc sp. Ft salt and Scytonema sp. F 18 against Aspergillus niger and Fusarium sp. Nostoc sp. F 3 and Nostoc sp. F 4 were the only strains that showed good activity against Bacillus cereus. Lastly, we conduct a NRPS PCR, using molecular techniques, in order to sequence and phylogenetic analysis the genes responsible for the production of secondary metabolites from ten cyanobacterial strains. Moreover, Adenylation domain substrate specificity predictions for NRPS enzymes, the signature sequence and the name of the compound, were made using NRPSpreditor 2 . Results:Sequence analysis indicates the enzymes encoded by these genes may be responsible for the production of different secondary metabolites, such as antibiotics. The sequences of the genes presented in this study have been deposited in GenBank. Conclusion:The presented results prove that fresh water cyanobacterial of Iran are a promising source to yield chemical and pharmaceutical interesting compounds.کلیدواژه ها
Peptide synthetase genes (NRPSs), Fresh water cyanobacterial, Genetic analysisمقالات مرتبط جدید
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