PHENOTYPIC DETECTION OF ANTIBIOTIC RESISTANCE AND EXTENDED SPECTRUM Β-LACTAMASE IN ECOLI STRAIN ISOLATES IN TABRIZ SINA HOSPITAL

Background and Aim:Production of β-lactamase enzymes by Gram-negative bacteria is the most common mechanism to acquire drug resistance to β-lactam antibiotics. This study was carried out to determine the frequency of occurrence and the antimicrobial susceptibility pattern of ESBL producing E. coli species from clinical isolates at Sina hospital in Tabriz.Methods:This cross- sectional study was carried out in Tabriz sina hospital during 2017 .A total of 100 ecoli samples were selected randomly. The isolates, identification was done by biochemical method. Antibiotic resistance of isolates was detected by disk diffusion method. These multidrug-resistant strains were phenotypically screened for ESBL production by phenotypic confirmatory disc diffusion test and double disc synergy test.Results:Among the 100 isolates, 49 were ESBL producers. All ESBL producers were highly resistant to Ampicilin (91%), cotrimoxazole (72%), and Ceftazidime (65%). However, these bacterial strains were sensitive to Ciprofloxacin (76%) and Amikacin(71%).Conclusion:This study showed that ESBL producing organisms were not only resistant to cephalosporins but also to other group of drugs and also that multiple mechanisms play a role in drug resistance among Gram-negative bacteria.

کلیدواژه ها:

Extended spectrum β-lactamase ، Eschersia coli ، Antibiotic resistance