EVALUATION OF ANTIBACTERIAL ACTIVITY OF HYDRO-ALCOHOLIC EXTRACT IN HERBAL PLANT SPECIES, NATIVE OF FASA, FARS

Background and Aim:The excessive use of antibiotics has led to increase in drug resistance in most pathogens. Research shows that medicinal plants and their natural derivatives can have similar effects to synthetic drugs; the aim of this study was evaluate the antibacterial activity of hydro-alcoholic extract of ten native herbs of Fasa, Fars province.Methods:In this study, antibacterial effects of hydro-alcoholic extract (Punica granatum, Salvia officinalis, Zataria multiflora, Artemisia adamsii, Malva sylvestris, Peganum harmala, Heracleum persicum, Chamaemelum nobile, Trachyspermum, Achillea millefoliom) evaluared by well-established and disk diffusion methods. Experiments were carried out on S. aureus (PTCC 1431) and E. coli (PTCC 1399), three times. Disks containing 20, 30, 40 and 50 μl of plant extract according to standard antibiotic concentrations as well as wells created with a diameter of 6 mm with similar concentrations (Muller Hinton Agar); inhibition zone diameter of bacterial growth was measured accurately after 24 hrs.Results:Compared with standard antibiotic disks, the antibacterial effects against S. aureus indicate the highest inhibition zone in Punica granatum with 22 mm diameter (well method) and 20 mm in Peganum harmala (disk method); antibacterial effects against E. coli, in both methods related to Peganum harmala with 15 to 12 mm diameter, respectively. The lowest effect related to Salvia officinalis, Heracleum persicum and Achillea millefoliom.Conclusion:Considering the results in comparison with standard antibiotic disks, further research on the mechanism of the effect of these plants, extraction and purification of effective substances and determination of focal doses are undergoing.

کلیدواژه ها:

herbal plants ، Hydro-alcoholic extract ، Antibacterial ، Drug resistance ، Antibiotic