PREVALENCE OF TEM, CTX-M AND SHV GENES IN KLEBSIELLA PNEUMONIAE ISOLATED FROM PATIENTS WITH VENTILATOR-ASSOCIATED PNEUMONIA

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 571

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شناسه ملی سند علمی:

MEDISM19_192

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Ventilator-associated pneumonia (VAP) is one of the most common infections in intensive care unite with high mortality rate. The aim of this study was to determine the prevalence of TEM, CTX-M and SHV genes in Klebsiella pneumoniae isolated from Ventilator-associated pneumonia in Kermanshah.Methods:The study was carried out on Bronchoalveolar lavage (BAL) samples from hospitalized patients in intensive care unit. After samples collection, 57 Klebsiella pneumoniae isolates were confirmed by bacteriology and biochemistry tests. Then antibiotic sensitivity test was done by disk diffusion method and ESBL enzymes existence was phenotypically determined by combined disk method. The frequency of ESBL genes was determined by their specific primer and PCR method.Results:The highest antibiotic resistance in Klebsiella pneumoniae was to Ceftriaxone and cotrimoxazole (78.9%), whereas the lowest antibiotic resistance was to Colistin (3.5%) and Imipenem (33.3%). Genotypically the frequency of SHV, TEM and CTX-M genes was 18(31.6%),13 (22.8%) and 7(12.3%) Respectively.Conclusion:According to the high resistance of Klebsiella pneumoniae isolated from patient’s samples with Ventilator-associated pneumonia to third generation Cephalosporin and the prevalence of ESBL producing strain in these patients, identification of broad-spectrum beta-lactamase producing strain and selecting proper antibiotic seem to be necessary.

نویسندگان

Kamal Ahmadi

Ph.D. Student of Medical Bacteriology, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran

Gholamreza Yousefi-Fatmesari

Assistant Professor, Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Mohsen Azizi

MSc, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah,Iran

Alireza Janbakhsh

Associate Professor, Department of Infectious Disease, Kermanshah University of Medical Sciences,Kermanshah, Iran