EVALUATION OF ANTIBACTERIAL ACTIVITY OF MATHANOL,ETHYL ACETATE AND HEXANE EXTRACTS OF CRUCIATA TAURIACA

Background and Aim:Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics. The aim of this study was to evaluate the antibacterial activity of Cruciata Tauriaca extracts against two Gram-posetive bacterial species.Methods:This plant have been collected in spring. Methanol and n-hexan was used for extraction of plant polar and nonpolar materials. Then minimum inhibitory concentration (MIC) of the plant materials were determined against Bacillus cereus and Staphylococcus aureus by broth micro-dilution method. Mueller hinton broth was used for preparation of serial diluted samples which were checked against 0.5-1 × 106 CFU (Colony forming unit) of bacteria. Incubation was done for 20 hrs at 37oC. MICs were recorded as the lowest concentrations which could inhibit visible growth of bacterium. Chloramphenicol was used as standard antibiotic.Results:Methanolic extract of Cruciata Tauriaca could show the best result (MICs 0.467 mg/ml for Bacillus cereus) and Staphylococcus aureus was inhibited in higher concentration of methanolic extract. (3.74 mg/ml).Conclusion:According to the result of this study, methanolic and hexane extracts can exhibit antibacterial effects.

کلیدواژه ها:

Antibacterial activity ، MIC ، Cruciata Tauriaca