EVALUATION ANTIMICROBIAL SUSCEPTIBILITY PATTERNS AND PRESENCE OF ESBLS AMONG CLINICAL ISOLATES OF P. AERUGINOSA COMPARED TO THE ENVIRONMENTAL AND COCKROACH ISOLATES

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 405

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شناسه ملی سند علمی:

MEDISM19_130

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:The incidence of extended-spectrum β-lactamases (ESBLs) in Pseudomonas aeruginosa being increasingly reported globally. Knowledge of the prevalence of ESBLs enzymes among clinical isolates compared to the environmental and cockroach isolates is limited. Therefore, the aim of this study was to evaluate the antimicrobial susceptibility and the presence of ESBLs among clinical P. aeruginosa isolates compared to the environmental and cockroach isolatesMethods:A total of 34 clinical, 19 environmental, and 7 cockroaches isolates of the P. aeruginosa were collected from three hospitals in Hamadan, west of Iran, and identified via API 20NE. The antimicrobial patterns were tested by disk diffusion method as recommended by CLSI. ESBLs encoding genes included blaCTX-M, blaTEM, and blaSHV were amplified by PCR method.Results:Susceptibility pattern of P. aeruginosa isolates showed that, clinical isolates were highly resistant to imipenem 18 (52.9%) and ciprofloxacin 15 (44.1%). In the environmental isolates, the highest resistance rate was seen against imipenem 10 (52.6%) followed by ciprofloxacin 8 (42.1%). However, all cockroaches’ isolates were susceptible to all of studied antibiotics. The difference in resistance rates between the clinical, environmental and cockroach isolates were statistically significant (P> 0.05). The prevalence of blaCTX-M, blaTEM, and blaSHV among P. aeruginosa were 48.3%, 36.7% and 38.3%, respectively.Conclusion:Our data indicated a high prevalence of ESBLs among clinical and environmental P. aeruginosa isolates carrying blaTEM, blaCTX-M, and blaSHV genes. The presence of ESBL-producing bacteria within the healthcare setting in should be considered a public health concern, as it causes limitations to the antimicrobial agents for optimal treatment of patients.

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نویسندگان

Omid Zarei

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Farezeh Kosari

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Ali Mohammadi Bardbari

University of Medical Sciences, Shahrekord, Iran

Pezhman Karami

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran