IN SILICO IDENTIFICATION AND COMPARATIVE GENOMICS OF CANDIDATE GENES INVOLVED IN ‎BIOSYNTHESIS NATURAL PRODUCTS IN CYANOBACTERIA STRAINS OF IRAN

Background and Aim:Cyanobacteria have the ability to produce a wide variety of natural bioactive compounds. ‎Horizontal gene transfer is thought to play a role in the sporadic distribution of bioactive ‎compounds producers among cyanobacteria. The most prevalent cyanobacterial natural products ‎are produced in combinatorial pathways like mixed PKS/NRPS systems. Nonribosomal peptide ‎synthetases (NRPSs) and polyketide synthase (PKSs), synthesize a diverse array of bioactive ‎small peptides, many of which are used in medicine. Methylprolines are non proteinogenic amino ‎acids produced by cyanobacteria. ‎Methods:To investigation the relationship between natural product genes and the role of horizontal gene ‎transfer in the evolutionary history, we amplified and sequenced the NRPS, PKS and ‎Methylprolines biosynthetic genes from from 25 cyanobacteria strains isolated from different ‎Climatic/Geographic Regions and Habitats. The analyses were done using a combination of the ‎genome mining softwares, AntiSMASH, and NaPDoS. Moreover, adenylation domain substrate ‎specificity predictions for NRPS enzymes, the signature sequences and the name of the ‎compounds, were made using NRPSpreditor2. In addition; we detected Methylprolines ‎biosynthetic gene from 8 strains included in this study. ‎Results:Our results suggest that Methylprolines biosynthetic genes have a complex evolutionary history in ‎cyanobacteria, which has been punctuated by a series of ancient horizontal gene transfer events ‎and probably the ability of lateral gene transfer to produce the natural bioactive compound has ‎been lost during evolutionary history in these strains.‎Conclusion:The data presented here highlight the need for future studies to define the linkages between ‎horizontal gene transfers in maintenance of bioactive compounds genes.‎

کلیدواژه ها:

Prediction of substrate ، Adenylation domains ، NRPSs genes