INVESTIGATION OF THE PRESENCE OF EXOTOXIN S IN PSEUDOMONAS AERUGINOSA WITH BROAD-SPECTRUM BETA-LACTAMASE

Background and Aim:Pseudomonas aeruginosa is one of the important pathogens in hospitals. T3SS is a determining factor in the pathogenesis of Pseudomonas aeruginosa and is directly associated to the human infections. The aim of this study was to investigate the association between antibiotic resistance and the ability of bacteria to produce exo-enzyme S.Methods:In this study, 100 clinical isolates including 50 ESBL + isolates were studied. In order to demonstrate the presence of ESBL enzymes, a disk diffusion method was used on the Muller Agar media. Ceftazidime / Ceftazidime clavulanic acid and cefotaxime / cefotaxime clavulanic acid were used for this purpose. In the following, the presence of exoS genes was investigated using PCR method.Results:The obtained results showed the presence of exo-enzyme S in most clinical isolates with more antibiotic resistance pattern. The presence of exoS enzyme was obtained as 62.8%. Clinical isolates with ESBLs showed higher capability for production of Exoenzyme S.Conclusion:Establishment of secretion System type 3 among clinical isolates of Pseudomonas aeruginosa is an important factor in the development of human diseases. The results of our study indicate the activity of T3S among the majority of clinical isolates. However, further studies are required to determine the relationship between the production of each exogenous species and the type of infection, and as a result, demonstrating the role of T3S in the infection of of Pseudomonas aeruginosa is necessary.

کلیدواژه ها:

Exotoxin S ، Pseudomonas aeruginosa ، ESBLs