SPA TYPING OF STAPHYLOCOCCUS AUREUS ISOLATES CAUSING NOSOCOMIAL INFECTIONS

Background and Aim:Spa-typing, is a typing method based on the DNA sequence analysis of the protein A gene. The purpose of this study was to molecular typing of Staphylococcus aureus isolated from patients in Toohid and Besat hospitals Sanandaj (2014).Methods:The clinical specimens from hospitalized patients were collected over a period of 1 year. Staphylococcus aureus isolates were identified by culture and biochemical standard methods based on CLSI guideline. Spa gene patterns in Staphylococcus aureus isolates were identified spa-typing techniques.Results:In total, 20 different patterns of Spa gene in staphylococcus aureus isolates were obtained in this study, which includes 6 type t030, 3 type (t230, t459 & t701), 2 type (t11332 & t304) and types t325, t012, t1149, t1810, t197, t325, t7789, t808, t871, t937, t14896, t14913, t14928 and t14929. The highest prevalence was belong to types t030 (18.18%), type t230, t459 and t701 (9.09%). New types t14896, t14913, t14928 & t14929 were identified during this study.Conclusion:In conclusion there was a well-known pattern of Spa types and also we identified new types that require more studies to qualify. Analysis of these patterns can improve insight to design nosocomial infection control programs.

کلیدواژه ها:

Staphylococcus aureus ، Spa Typing ، Sanandaj ، Epidemiology