Hydrocortisone Modulates Junctional Molecules of Human Endometrial Epithelial Cells

The embryo implantation process in mammals is considered a remarkable evolutionary strategy for pregnancy success and endometrial receptivity should be established for successful implantation. Breakdown of the endometrial epithelial cell barrier is a key event during embryo implantation. Recently it has been shown that corticosteroidssuch as hydrocortisone (HC) increase the tight junction molecules expression in epithelial barriers. In our study, we investigated whether HC increase the tight junction molecules expression in human endometrial epithelial cells. The candidate genes were Zona occludin-1(ZO-1), Claudin4, Claudin3, Desmogelin and E-cadherin. Humanendometrial epithelial cells were cultured in four concentration of hydrocortisone (0, 50, 100 and 200nM) by three durations (24, 48 and 72h). The expression of tight junction molecules were investigated by QRT-PCR and compared to control. Our findings indicated that HC significantly enhances the expression of tight junction molecules in 100nM concentration after 48h treatment but decrease in 200nM concentration after 72h. Taken together, it seems HC by increasing the expression of tight junction molecules and enhancement of tight junction integrity can influence embryo implantation.

کلیدواژه ها:

Ectopic Pregnancy ، Human Fallopian Tube ، Hydrocortisone ، Tight Junction