The Effect of Seminal Plasma in Extenders Containing Glutamine and Proline on Oxidative Stress And Motility of Cooled Stallion Sperm

Preservation of cooled equine semen against oxidative stress and loss of motility might be improved by addition of specific components to extenders. This study tested the hypothesis that supplementation of extenders with proline and glutamine for cooling storage of equine spermatozoa in the presence of seminal plasma (SP) affects motility, lipid peroxidation and enzymatic antioxidant status. Ten semen samples from five fertile stallions diluted in basal medium (E1), containing 5mM glutamine (E2) and 3mMproline (E3) stored in 5 for analysis of motion parameters at 1.5, 24 and 48 hours storage. Sperm pellet after SP removal resuspended in basal medium (E4), containing 5mM glutamine (E5) and 3mM proline (E6) stored in 5°C for 1.5 hours to determine malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX) and motility . The motion parameters of E1, E2 and E3 didn’t change over time. At 1.5 hours of storage, there wasn’t significant difference in motion parameters of E4, E5 and E6 but motility reduced in E3. In E2 and E3 MDA decreased, CAT increased and GPX remained unchanged. In E5 and E6 MDA decreased but CAT and GPX decreased with E5. The present study revealedthat in the absence of SP, motion parameters were not affected by aminoacids. In the presence of SP, glutamine and proline significantly increased CAT level of cooled sperm and proline significantly reduced motility. In conclusion negative effects of proline on motion parameters seems to be related to interaction with SP which is completely irrelevant to lipid peroxidation and antioxidative pathways.

کلیدواژه ها:

Stallion ، Sperm ، Seminal Plasma ، Glutamine ، Proline ، Oxidative Stress ، Cooling Storage