Proliferative Ability of Human Spermatogonial Stem Cells in in-vitro condition

Background: The primordial spermatogonia can resume spermatogenesis process after testicles damages caused by poisonous materials or after their cell fusion with an infertile receptor cell. Therefore, the self-renewal of these cells guarantees the preservation of their cell population and results in protection of fertility. Distinction of primordial spermatogonia markers makes the separation of this cell population possible . Preservation and cell culture of the primordial spermatogonia has been propounded as a method to treat some infertility disorders in human in the future.Methods: Isolation and purification of human spermatogonial stem cells cultured in viroResult: molecular mechanisms of self-renewal, a perfect tool to discover new tretments for some infertile men or for patients undergoing chemotherapy /radiotheraoy, before or after puberty.Conclusion: Spermatogenesis by endocrine factors and growth factors autocrine / paracrine testicular set. These factors Sertoli cells, germ cells, the tubular cells and interstitial cells (mainly Alydyg cells and macrophages) are produced. And the interaction between Sertoli cells and germ cells in the seminiferous tubules, causing succeeds in spermatogenesis. Recently, a number of important growth factors such as LIF, SCF and GDNF for proliferation and differentiation of spermatogonial stem cells, have been identified. As well as markers to identify these cells have been reported. Several research groups in the field of long-term cultivation and enrichment of spermatogonial stem cells to have gained some success. Therefore, the cultivation of spermatogonial stem cells, in addition to helping understand the molecular mechanisms of self-renewal, a perfect tool to discover new treatments for some infertile men or for patients undergoing chemotherapy / radiotherapy, before or after puberty

کلیدواژه ها:

spermatogonial ، cell culture