The effects of human amniotic epithelial cells on naïve CD4+, CD25 - T cells activation from unexplained recurrent spontaneous abortion patients
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_342
- زبان مقاله: انگلیسی
- تعداد مشاهده: 431
نویسندگان
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Immunology Research Center (IRC), Iran University of Medical Sciences, Tehran, Iran
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Background: The unexplained recurrent spontaneous abortion (URSA) is a common disorder in 1%–5% of women of reproductive age. Several treatments have been used for URSA; however, all are controversial. As human amniotic epithelial cells (hAECs) have immunomodulatory properties, in this study, we investigated immunomodulatory effects of hAECs on activation of naïve T cells from USRA patients.Methods: Naïve CD4+, CD25- T cells isolated from 25 patients with URSA using MACS technique. hAECs were separated from amnion delivered by healthy women with uncomplicated pregnancies during elective cesarean deliveries. Naive T cells (4 × 10 5) were co-cultured at different ratios with hAECs (1:1, 1:2, 1:5, 1:10) along with the positive and negative control (1:0) for 3 and 6 days. Naïve T cells were activated with anti CD3/CD28 (1μg/ml) and INF-γ secretion of activated T cells was assessed in culture supernatant by ELISA.Result: IFN-γ level in 72h culture supernatant at 1:1, 1:2, 1:5 ratios was significantly lower than positive control (pConclusion: These findings suggest that hAECs have suppressive activities on naïve T cells activation from URSA patients in vitro. These suppressive effects were time-dependant and often perform through releasing suppressive mediators. Thus, it seems that hAECs may be a potential cell source as therapy for URSA.کلیدواژه ها
Immunomodulatory effects., Naïve T cells, Recurrent spontaneous abortion, Amniotic Epithelial Cellsاطلاعات بیشتر در مورد COI
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