CRISPR-Cas: A new technology for studding genes playing a role in reproduction
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_304
- زبان مقاله: انگلیسی
- تعداد مشاهده: 627
نویسندگان
Immunology research center, Iran University of Medical Sciences, Tehran, Iran
Immunology research center, Iran University of Medical Sciences, Tehran, Iran
Islamshahr Azad University, Islamshahr, Tehran, Iran
Islamshahr Azad University, Islamshahr, Tehran, Iran
چکیده
Background: CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated) is a new tool for genome editing which is modified from an immune system in prokaryotes against viruses and plasmid. CRISPR-Cas system works by introducing a site specific double strand cut in DNA which allows existing genes to be removed or new one added. This technology can also be used to create mouse models carrying a special insertion, deletion or point mutation much easier. In this way CRISPR-Cas system can be used for studding different genes and molecular mechanisms which play a role in male germ cells. For example zfy1/2 double knockout mouse have shown infertility and abnormal sperm morphology. In this review, we will describe how CRISPR-Cas system works and will review some of the studies which have used this system to explore the role of some genes in spermatogenesis, infertility and reproduction.Methods: Relevant literature was identified by a PubMed search of English-language literature using the terms CRISPR-Cas, genome editing, reproduction and spermatogenesis.Result: The role of different genes in spermatogenesis, infertility and reproduction could have been successfully studied using CRISPR-Cas system.Conclusion: CRISPR-Cas is a fast technique to make knockout or mutated mouse models and studding and editing genome.کلیدواژه ها
Genome editing, Reproduction, Spermatogenesis, CRISPR-Casاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.